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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleRole Of Cyclic Gmp On Inhibition By Nitric Oxide Donors Of Human Eosinophil Chemotaxis In Vitro.pt_BR
dc.contributor.authorThomazzi, Sara Mpt_BR
dc.contributor.authorMoreira, Julianapt_BR
dc.contributor.authorMarcondes, Sisipt_BR
dc.contributor.authorDe Nucci, Gilbertopt_BR
dc.contributor.authorAntunes, Edsonpt_BR
unicamp.authorSara M Thomazzi, Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, PO Box 6111, 13084-971, Campinas (SP), Brazil. thomazzi@directnet.com.brpt_BR
unicamp.author.externalJuliana Moreira,pt
unicamp.author.externalSisi Marcondes,pt
unicamp.author.externalGilberto De Nucci,pt
unicamp.author.externalEdson Antunes,pt
dc.subjectAdolescentpt_BR
dc.subjectAdultpt_BR
dc.subjectBlotting, Westernpt_BR
dc.subjectCell Survivalpt_BR
dc.subjectChemotaxis, Leukocytept_BR
dc.subjectCyclic Gmppt_BR
dc.subjectEosinophilspt_BR
dc.subjectFemalept_BR
dc.subjectHumanspt_BR
dc.subjectIn Vitro Techniquespt_BR
dc.subjectMalept_BR
dc.subjectMiddle Agedpt_BR
dc.subjectMolsidominept_BR
dc.subjectN-formylmethionine Leucyl-phenylalaninept_BR
dc.subjectNitric Oxide Donorspt_BR
dc.subjectNitroprussidept_BR
dc.subjectPenicillaminept_BR
dc.subjectTetrazolium Saltspt_BR
dc.subjectThiazolespt_BR
dc.subjectTyrosinept_BR
dc.description.abstract1. This study was designed to investigate the effects of the nitric oxide (NO) donors sodium nitroprusside (SNP), 3-morpholinosydnonimine (SIN-1) and S-nitroso-N-acetylpenicillamine (SNAP) on N-formyl-L-methionyl-L-leucyl-phenylalanine (fMLP, 1 x 10(-7) M)-induced human eosinophil chemotaxis, cyclic guanosine-3',5'-monophosphate (cGMP) levels, protein nitration and cytotoxicity. 2. Human eosinophils were exposed to SNP, SIN-1 and SNAP (0.001-1.0 mM) for either short (10 min) or prolonged (90 min) time periods. Exposition of eosinophils with these NO donors significantly inhibited the eosinophil chemotaxis irrespective of whether cells were exposed to these agents for 10 or 90 min. No marked differences were detected among them regarding the profile of chemotaxis inhibition. 3. Exposition of eosinophils to SNP, SIN-1 and SNAP (0.001-1.0 mM) markedly elevated the cGMP levels above basal levels, but the 90-min exposition resulted in significantly higher levels compared with the 10-min protocols (5.3+/-0.6 and 2.6+/-0.2 nM 1.5 x 10(6) cells(-1), respectively). The cGMP levels achieved with SNAP were greater than SNP and SIN-1. 4. The NO donors did not induce cell toxicity in any experimental condition used. Additionally, eosinophils exposed to SNP, SIN-1 and SNAP (1.0 mM each) either for 10 or 90 min did not show any tyrosine nitration in conditions where a strong nitration of bovine serum albumin was observed. 5. Our findings show that inhibitory effects of fMLP-induced human eosinophil chemotaxis by NO donors at short or prolonged exposition time were accompanied by significant elevations of cGMP levels. However, additional elevations of cGMP levels do not change the functional profile (chemotaxis inhibition) of stimulated eosinophils.en
dc.relation.ispartofBritish Journal Of Pharmacologypt_BR
dc.relation.ispartofabbreviationBr. J. Pharmacol.pt_BR
dc.date.issued2004-Febpt_BR
dc.identifier.citationBritish Journal Of Pharmacology. v. 141, n. 4, p. 653-60, 2004-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume141pt_BR
dc.description.firstpage653-60pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0007-1188pt_BR
dc.identifier.doi10.1038/sj.bjp.0705661pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/14744805pt_BR
dc.date.available2015-11-27T12:58:20Z-
dc.date.accessioned2015-11-27T12:58:20Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:58:20Z (GMT). No. of bitstreams: 1 pmed_14744805.pdf: 188314 bytes, checksum: 8aceec51cc6722403eac70c7b9dab453 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195888-
dc.identifier.idPubmed14744805pt_BR
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