Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195880
Type: Artigo de periódico
Title: Effect Of Hypoxia On Macrophage Infection By Leishmania Amazonensis.
Author: Colhone, Marcelle Carolina
Arrais-Silva, Wagner Welber
Picoli, Claudia
Giorgio, Selma
Abstract: In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with gamma-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection.
Subject: Animals
Cell Hypoxia
Cell Line
Cell Survival
Cells, Cultured
Humans
Interferon-gamma
Leishmania Mexicana
Lipopolysaccharides
Macrophages, Peritoneal
Mice
Mice, Inbred Balb C
Nitric Oxide
U937 Cells
Rights: fechado
Identifier DOI: 10.1645/GE-3286
Address: http://www.ncbi.nlm.nih.gov/pubmed/15270094
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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