Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195858
Type: Artigo de periódico
Title: Pharmacological And Histopathological Characterization Of Bothrops Lanceolatus (fer De Lance) Venom-induced Edema.
Author: Guimarães, A Q
Cruz-Höfling, M A
Ferreira de Araújo, P M
Bon, C
Lôbo de Araújo, A
Abstract: Bothrops venoms cause local edema, pain, hemorrhage and necrosis. In this study, we investigated the ability of Bothrops lanceolatus venom to cause edema in rat hind paws and examined the mediators involved. Hind paw edema was induced in male Wister rats by the subplantar injection of venom (12.5-100 microg/paw) in the absence and presence of antagonists. Edema was quantified by hydroplethysmometry at 0.25, 0.5, 2, 4, 6 and 24 h post-injection and was expressed as the percentage increase relative to the contralateral (control) paw. The ability of the venom to release histamine from rat peritoneal mast cells was also assessed. Venom caused dose- and time-dependent edema that was maximal within 15 min but disappeared after 24 h and was accompanied by hemorrhage. Dexamethasone (1 mg/kg, s.c.), methysergide (6 mg/kg, i.p.), HOE 140 (0.6 mg/kg, i.v.) and mepyramine (6 mg/kg, i.p.) significantly ( p < 0.05) reduced edema formation, whereas indomethacin (10 mg/kg, i.p.) was ineffective. Dialysis did not affect venom-induced edema. Venom (1, 10 and 30 microg/ml) caused a concentration-dependent release of histamine (13 +/- 1%, 61.9 +/- 4.6% and 73.6 +/- 2.4%, respectively; n = 5) from rat peritoneal mast cells in vitro. Histological analysis confirmed the presence of edema, hemorrhage and neutrophil infiltration. Pretreating the venom with EDTA partially inhibited the edema and hemorrhage, but did not affect the migration of neutrophils. B. lanceolatus venom produced dose- and time-dependent edema in rat paws. This edema was not dependent on low molecular weight substances in the venom, but was partially dependent on a hemorrhagin and also involved the release of arachidonic acid metabolites, bradykinin, histamine and serotonin.
Subject: Adrenergic Beta-antagonists
Animals
Antiemetics
Arachidonic Acid
Bothrops
Bradykinin
Cardiovascular Agents
Crotalid Venoms
Dexamethasone
Dose-response Relationship, Drug
Edema
Edetic Acid
Extremities
Histamine
Histamine H1 Antagonists
Indomethacin
Mast Cells
Methysergide
Pyrilamine
Rats
Rats, Wistar
Serotonin
Time Factors
Vasoconstrictor Agents
Rights: fechado
Identifier DOI: 10.1007/s00011-004-1258-0
Address: http://www.ncbi.nlm.nih.gov/pubmed/15241562
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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