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dc.typeArtigo de periódicopt_BR
dc.titleFatty Acid Synthase Is Required For The Proliferation Of Human Oral Squamous Carcinoma Cells.pt_BR
dc.contributor.authorAgostini, Michellept_BR
dc.contributor.authorSilva, Sabrina Dpt_BR
dc.contributor.authorZecchin, Karina Gpt_BR
dc.contributor.authorColetta, Ricardo Dpt_BR
dc.contributor.authorJorge, Jackspt_BR
dc.contributor.authorLoda, Massimopt_BR
dc.contributor.authorGraner, Edgardpt_BR
unicamp.authorMichelle Agostini, Department of Oral Diagnosis, School of Dentistry of Piracicaba, UNICAMP, Av. Limeira 901, CP 52, Areão, SP 13414-018, Brazil.pt_BR D Silva,pt G Zecchin,pt D Coletta,pt Jorge,pt Loda,pt Graner,pt
dc.subjectCarcinoma, Squamous Cellpt_BR
dc.subjectCell Divisionpt_BR
dc.subjectEpidermal Growth Factorpt_BR
dc.subjectFatty Acid Synthasespt_BR
dc.subjectMouth Neoplasmspt_BR
dc.subjectReceptor, Epidermal Growth Factorpt_BR
dc.subjectReceptor, Erbb-2pt_BR
dc.subjectReceptors, Androgenpt_BR
dc.subjectReverse Transcriptase Polymerase Chain Reactionpt_BR
dc.subjectTumor Cells, Culturedpt_BR
dc.description.abstractFatty acid synthase (FAS) is the enzyme responsible for the endogenous synthesis of saturated long-chain fatty acids from the precursors acetyl-CoA and malonyl-CoA. A growing body of evidence indicates that FAS is over expressed in several human cancers, such as prostate, breast, bladder, liver, lung, melanoma and oral squamous cell carcinoma (SCC). In the present study we used human oral SCC cell lines (SCC-4, -9, -15 and -25) as a model to investigate the role of FAS in the pathogenesis of oral cancer. RT-PCR and western blot experiments demonstrated that FAS is differentially expressed by the four oral SCC cell lines, with the highest production in SCC-9 followed by SCC-25. FAS expression in SCC-4 and -15 was similarly lower than the other cell lines. Proliferation curves and immunocytochemistry for PCNA and Ki-67 demonstrated that SCC-25 has the highest proliferative potential. In addition, the specific inhibitor of FAS activity cerulenin was able to significantly reduce the proliferation of oral SCC cells. Expression of androgen receptor was low in SCC-4, -9 and -15 and undetectable in SCC-25, whereas EGFR and c-erb-B2 were expressed in high amounts by the four cell lines. Immunocytochemical reactions showed that SCC-25 expresses higher levels of EGF compared to the other three cell lines. Finally, oral SCC cells exposed to nanomolar concentrations of exogenous EGF presented a reduction in the FAS protein levels concomitant with a decrease in their proliferation rates. Taken together, our results indicate that FAS is expressed in an apparently androgen-independent fashion in oral SCC cells and it is necessary for their proliferation.en
dc.relation.ispartofOral Oncologypt_BR
dc.relation.ispartofabbreviationOral Oncol.pt_BR
dc.identifier.citationOral Oncology. v. 40, n. 7, p. 728-35, 2004-Aug.pt_BR
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