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Type: Artigo de periódico
Title: Detection Of Bovine Respiratory Syncytial Virus In Experimentally Infected Balb/c Mice.
Author: Almeida, Renata Servan
Domingues, Helena Gallicchio
Coswig, Lia Treptow
D'Arce, Regina Celia Freitas
de Carvalho, Rodrigo Franco
Arns, Clarice Weis
Abstract: The present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. As a first step, Chicken Embryo Related (CER) cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author's knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb/c mice after experimental inoculation.
Subject: Animals
Cattle Diseases
Chick Embryo
Dna, Viral
Mice, Inbred Balb C
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Bovine
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity And Specificity
Specific Pathogen-free Organisms
Citation: Veterinary Research. v. 35, n. 2, p. 189-97
Rights: aberto
Identifier DOI: 10.1051/vetres:2004003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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