Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Dna Content, Chromatin Texture And Nuclear Morphology In Benzo[a]pyrene-transformed Human Breast Epithelial Cells After Microcell-mediated Transfer Of Chromosomes 11 And 17.
Author: Mello, Maria Luiza S
de Campos Vidal, Benedicto
Lareef, Mohamed H
Hu, Yun F
Yang, Xiaoqi
Russo, Jose
Abstract: A relation between the changes in DNA content and chromatin supra-organization and the expression of gradual steps of tumorigenesis has been assessed by image analysis in human breast epithelial cells (MCF-10F) treated with benzo[a]pyrene (BP) (cell lines BP1, BP1-E, BP1-Tras, and others). Because abnormal chromosomes 11 and 17 have been associated with neoplastic progression in BP-transformed MCF-10F cells, image analysis of Feulgen-stained tumorigenic BP1-E cells with the microcell-mediated chromosome transfer of normal chromosomes 11 and 17 was carried out. A tendency of DNA amount distribution and nuclear size restoration to values typical of non-transformed MCF-10F cells was demonstrated, especially after the transfer of chromosome 17. No reversion in chromatin texture was found after the transfer of chromosome 11 or 17. Although the presence of a normal chromosome 17 should be considered among the necessary steps for tumorigenic human breast epithelial cells to recover their normality, a more complex genome balance is required for the entire nuclear chromatin of these cells to recover its totally normal supra-organization and expression.
Subject: Benzo(a)pyrene
Cell Line, Transformed
Cell Nucleus
Cell Transformation, Neoplastic
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 17
Epithelial Cells
Gene Transfer Techniques
Image Cytometry
Microscopy, Video
Citation: Cytometry. Part A : The Journal Of The International Society For Analytical Cytology. v. 52, n. 2, p. 70-6, 2003-Apr.
Rights: fechado
Identifier DOI: 10.1002/cyto.a.10029
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_12655650.pdf438.33 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.