Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195652
Type: Artigo de periódico
Title: Molecular Mapping In Tropical Maize (zea Mays L.) Using Microsatellite Markers. 1. Map Construction And Localization Of Loci Showing Distorted Segregation.
Author: Sibov, Sérgio Tadeu
de Souza, Cláudio Lopes
Garcia, Antonio Augusto Franco
Garcia, Alexandre Franco
Silva, Adelmo Rezende
Mangolin, Claudete Aparecida
Benchimol, Luciana Lasry
de Souza, Anete Pereira
Abstract: Microsatellites have become the most important class of markers for mapping procedures. Primarily based on restriction fragment length polymorphism (RFLP) markers, several molecular genetic maps of maize have been developed, mainly using temperate inbred maize lines. To characterize the level of polymorphism of microsatellite loci and construct a genetic map in tropical maize, two elite inbred lines, L-08-05F and L-14-4B, were crossed to produce 400 F(2) individuals that were used as a mapping population. A survey of 859 primer pair sequences of microsatellites was used. The polymorphism screens of each microsatellite and genotype assignment were performed using high-resolution agarose gels. About 54 % of the primer sets gave clearly scorable amplification products, 13 % did not amplify and 33 % could not be scored on agarose gels. A total of 213 polymorphic markers were identified and used to genotype the mapping population. Among the polymorphic markers, 40 showed loci deviating from expected Mendelian ratios and clusters of deviating markers were located in three chromosome regions. Non-Mendelian scoring was present in 19 markers. The final genetic map with 117 markers spanned 1634 cM in length with an average interval of 14 cM between adjacent markers.
Subject: Genetic Linkage
Genetic Markers
Microsatellite Repeats
Polymorphism, Genetic
Zea Mays
Rights: aberto
Identifier DOI: 10.1111/j.1601-5223.2003.01666.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/15061810
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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