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Type: Artigo de periódico
Title: Strongyloides Venezuelensis Alkaline Extract For The Diagnosis Of Human Strongyloidiasis By Enzyme-linked Immunosorbent Assay.
Author: Machado, Eleuza Rodrigues
Ueta, Marlene Tiduko
de Fátima Gonçalves-Pires, Maria do Rosário
Alves de Oliveira, João Batista
Faccioli, Lúcia Helena
Costa-Cruz, Julia Maria
Abstract: The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was >80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.
Subject: Animals
Antibodies, Helminth
Antigens, Helminth
Case-control Studies
Enzyme-linked Immunosorbent Assay
Rats, Wistar
Sensitivity And Specificity
Rights: aberto
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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