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Type: Artigo de periódico
Title: Human Leukocyte Formin: A Novel Protein Expressed In Lymphoid Malignancies And Associated With Akt.
Author: Favaro, Patricia M Bergamo
de Souza Medina, Samuel
Traina, Fabíola
Bassères, Daniela Sanchez
Costa, Fernando Ferreira
Saad, Sara Teresinha Olalla
Abstract: The very large family of Formin proteins is involved in processes such as morphogenesis, embryonic differentiation, cell polarity, and cytokinesis. A novel human gene from the Formin family, denominated human leukocyte formin gene, was cloned. The cDNA of the gene was determined to be 3959bp long with an open reading frame of 3302bp and computational analysis located this gene on chromosome 17, suggesting that it is composed of 27 exons. Northern blot analysis revealed a restricted expression of mRNA in the thymus, spleen, and peripheral blood leukocytes in normal human tissues. Western blot analysis demonstrated that the protein encoded by this gene is overexpressed in lymphoid malignancies; cancer cell lines and peripheral blood leukocyte from chronic lymphocytic leukemia (CLL) patients. Furthermore, the human leukocyte formin protein was observed to associate with Akt, a critical survival regulator in many different cell types.
Subject: Amino Acid Sequence
Cell Line, Tumor
Cytoskeletal Proteins
Gene Expression Regulation, Neoplastic
Hl-60 Cells
Jurkat Cells
Leukemia, Lymphocytic, Chronic, B-cell
Leukocytes, Mononuclear
Molecular Sequence Data
Protein-serine-threonine Kinases
Proto-oncogene Proteins
Proto-oncogene Proteins C-akt
Sequence Analysis, Protein
Rights: fechado
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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