Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195582
Type: Artigo de periódico
Title: Simple Fluorescent Pcr Method For Detection Of Large Deletions In The Beta-globin Gene Cluster.
Author: De Andrade, Tiago Gomes
Saad, Sara Teresinha Olalla
Sonati, Maria de Fatima
Costa, Fernando Ferreira
Abstract: We developed a semi-automated approach to detect large deletions in the beta-globin gene cluster, based on the quantitative differences in the amplifications of samples by a fluorescent PCR. With this strategy, we were able to detect the presence of HPFH-2 in an African-Brazilian subject, confirmed by sequencing analysis. Differently from other PCR strategies, GAP-PCR for example, it has the potential to identify new deletions.
Subject: Africa
Brazil
Fetal Hemoglobin
Fluorescent Dyes
Gene Deletion
Globins
Hematologic Diseases
Humans
Polymerase Chain Reaction
Thalassemia
Rights: fechado
Identifier DOI: 10.1002/ajh.10291
Address: http://www.ncbi.nlm.nih.gov/pubmed/12605399
Date Issue: 2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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