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Type: Artigo de periódico
Title: Acetylcholine Receptor Distribution And Synapse Elimination At The Developing Neuromuscular Junction Of Mdx Mice.
Author: Minatel, Elaine
Neto, Humberto Santo
Marques, Maria Julia
Abstract: The pattern of innervation of the vertebrate neuromuscular junction is established during early development, when junctions go from multiple to single innervation in the phenomenon of synapse elimination, suggesting that changes at the molecular level in the postsynaptic cell lead to the removal of nerve terminals. The mdx mouse is deficient in dystrophin and associated proteins that are part of the postsynaptic cytoskeleton. We used rhodamine-alpha-bungarotoxin and anti-neurofilament IgG-FITC to stain acetylcholine receptors and nerve terminals of the sternomastoid muscle during postnatal development in mdx and control C57BL/10 mice. Using fluorescence confocal microscopy, we observed that, 7 days after birth, 86.7% of the endplates of mdx mice were monoinnervated (n = 200) compared with 41.4% in control mice (n = 200). By the end of the second postnatal week, all endplates were innervated singly (100% mdx and 94.7% controls, n = 200 per group). These results show that dystrophic fibers achieve single innervation earlier, perhaps because dystrophin or a normal cytoskeletal complex is implicated in this phenomenon.
Subject: Animals
Animals, Newborn
Mice, Inbred C57bl
Mice, Inbred Mdx
Neuromuscular Junction
Receptors, Cholinergic
Citation: Muscle & Nerve. v. 28, n. 5, p. 561-9, 2003-Nov.
Rights: fechado
Identifier DOI: 10.1002/mus.10416
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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