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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleProteome Analysis Of The Plant Pathogen Xylella Fastidiosa Reveals Major Cellular And Extracellular Proteins And A Peculiar Codon Bias Distribution.pt_BR
dc.contributor.authorSmolka, Marcus Bustamantept_BR
dc.contributor.authorMartins-de-Souza, Danielpt_BR
dc.contributor.authorMartins, Danielpt_BR
dc.contributor.authorWinck, Flavia Vischipt_BR
dc.contributor.authorSantoro, Carlos Eduardopt_BR
dc.contributor.authorCastellari, Rafael Ramospt_BR
dc.contributor.authorFerrari, Fernandapt_BR
dc.contributor.authorBrum, Itaraju Juniorpt_BR
dc.contributor.authorGalembeck, Eduardopt_BR
dc.contributor.authorDella Coletta Filho, Helvéciopt_BR
dc.contributor.authorMachado, Marcos Antoniopt_BR
dc.contributor.authorMarangoni, Sergiopt_BR
dc.contributor.authorNovello, Jose Camillopt_BR
unicamp.authorMarcus Bustamante Smolka, Departamento de Bioquimica, UNICAMP, Campinas, SP, Brazil.pt_BR
unicamp.author.externalDaniel Martins-de-Souza,pt
unicamp.author.externalDaniel Martins,pt
unicamp.author.externalFlavia Vischi Winck,pt
unicamp.author.externalCarlos Eduardo Santoro,pt
unicamp.author.externalRafael Ramos Castellari,pt
unicamp.author.externalFernanda Ferrari,pt
unicamp.author.externalItaraju Junior Brum,pt
unicamp.author.externalEduardo Galembeck,pt
unicamp.author.externalHelvécio Della Coletta Filho,pt
unicamp.author.externalMarcos Antonio Machado,pt
unicamp.author.externalSergio Marangoni,pt
unicamp.author.externalJose Camillo Novello,pt
dc.subjectAntioxidantspt_BR
dc.subjectBacterial Adhesionpt_BR
dc.subjectBacterial Proteinspt_BR
dc.subjectCodonpt_BR
dc.subjectDatabases As Topicpt_BR
dc.subjectElectrophoresis, Gel, Two-dimensionalpt_BR
dc.subjectElectrophoresis, Polyacrylamide Gelpt_BR
dc.subjectGammaproteobacteriapt_BR
dc.subjectGenome, Bacterialpt_BR
dc.subjectImage Processing, Computer-assistedpt_BR
dc.subjectIronpt_BR
dc.subjectMass Spectrometrypt_BR
dc.subjectOpen Reading Framespt_BR
dc.subjectPeptidespt_BR
dc.subjectPlant Diseasespt_BR
dc.subjectPorinspt_BR
dc.subjectProteomept_BR
dc.description.abstractThe bacteria Xylella fastidiosa is the causative agent of a number of economically important crop diseases, including citrus variegated chlorosis. Although its complete genome is already sequenced, X. fastidiosa is very poorly characterized by biochemical approaches at the protein level. In an initial effort to characterize protein expression in X. fastidiosa we used one- and two-dimensional gel electrophoresis and mass spectrometry to identify the products of 142 genes present in a whole cell extract and in an extracellular fraction of the citrus isolated strain 9a5c. Of particular interest for the study of pathogenesis are adhesion and secreted proteins. Homologs to proteins from three different adhesion systems (type IV fimbriae, mrk pili and hsf surface fibrils) were found to be coexpressed, the last two being detected only as multimeric complexes in the high molecular weight region of one-dimensional electrophoresis gels. Using a procedure to extract secreted proteins as well as proteins weakly attached to the cell surface we identified 30 different proteins including toxins, adhesion related proteins, antioxidant enzymes, different types of proteases and 16 hypothetical proteins. These data suggest that the intercellular space of X. fastidiosa colonies is a multifunctional microenvironment containing proteins related to in vivo bacterial survival and pathogenesis. A codon usage analysis of the most expressed proteins from the whole cell extract revealed a low biased distribution, which we propose is related to the slow growing nature of X. fastidiosa. A database of the X. fastidiosa proteome was developed and can be accessed via the internet (URL: www.proteome.ibi.unicamp.br).en
dc.relation.ispartofProteomicspt_BR
dc.relation.ispartofabbreviationProteomicspt_BR
dc.date.issued2003-Febpt_BR
dc.identifier.citationProteomics. v. 3, n. 2, p. 224-37, 2003-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume3pt_BR
dc.description.firstpage224-37pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1615-9853pt_BR
dc.identifier.doi10.1002/pmic.200390031pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12601815pt_BR
dc.date.available2015-11-27T12:52:30Z-
dc.date.accessioned2015-11-27T12:52:30Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:52:30Z (GMT). No. of bitstreams: 1 pmed_12601815.pdf: 229752 bytes, checksum: 376f5a2f30cc9cf28e09d613e8a89bfa (MD5) Previous issue date: 2003en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195560-
dc.identifier.idPubmed12601815pt_BR
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