Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195536
Type: Artigo de periódico
Title: Effect Of Phosphate Concentration On The Production Of Dextransucrase By Leuconostoc Mesenteroides Nrrl B512f.
Author: Rodrigues, S
Lona, L M F
Franco, T T
Abstract: Leuconostoc mesenteroides NRRL B512F is the main strain used in industrial fermentations to produce dextransucrase and dextran. This process has been studied since the Second World War, when it was used as blood plasma expander. A study about the effect of phosphate concentration on cell propagation in a semicontinuous shake-flask culture is described in this work. Dextransucrase is obtained by fermentation of the Leuconostoc mesenteroides NRRL B512F in the presence of sucrose as substrate, a nitrogen source (corn liquor or yeast extract) and minerals. Phosphate is currently used in order to buffer the culture medium. Cell propagation can be done through a repeated batch culture, where dilution in a fresh medium is made with relatively short periods. The standard medium for dextransucrase production is prepared using 0.1 M of K(2)HPO(4). In this work the level of phosphate was increased to 0.3 M, and an increase on biomass and on the enzyme activity was found when phosphate enriched medium was used. Higher phosphate buffer concentration was also able to keep the pH values above 5.0 during the entire process, avoiding enzyme denaturation.
Subject: Bioreactors
Cell Culture Techniques
Cell Division
Computer Simulation
Dextrans
Dose-response Relationship, Drug
Enzyme Activation
Enzyme Stability
Glucosyltransferases
Hydrogen-ion Concentration
Leuconostoc
Models, Biological
Phosphates
Species Specificity
Rights: fechado
Identifier DOI: 10.1007/s00449-003-0330-4
Address: http://www.ncbi.nlm.nih.gov/pubmed/14505164
Date Issue: 2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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