Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195526
Type: Artigo de periódico
Title: Stimulation Of Potato Tuber Respiration By Cold Stress Is Associated With An Increased Capacity Of Both Plant Uncoupling Mitochondrial Protein (pump) And Alternative Oxidase.
Author: Calegario, Fagoni Fayer
Cosso, Ricardo Guanaes
Fagian, Marcia Marilia
Almeida, Fernanda Vasconcelos
Jardim, Wilson Figueiredo
Jezek, Petr
Arruda, Paulo
Vercesi, Anibal Eugênio
Abstract: The CO2 evolution of intact potato tubers (Solanum tuberosum, L., var. Bintje) was analyzed during a 10-day period of their warm (25 +/- 2 degrees C) or cold (5 +/- 1 degrees C) storage, to evaluate cold-stress effects on expression and activities of plant uncoupling mitochondrial protein (PUMP) and alternative oxidase (AOX). CO2 evolution rates were analyzed at 20 degrees C, to reflect their possible capacities. The 20 degrees C CO2 production declined from 13 to 8 mg kg(-1) h(-1) after 2 days of warm storage and then (after 3 to 7 days) decreased from 8 to 6.5 mg kg(-1) h(-1). In contrast, 20 degrees C CO2 evolution did not change after the first day of cold storage, increased up to 14.5 mg kg(-1) h(-1) after 2 days, and decreased to about 12 mg kg(-1) h(-1) after 3 to 7 days of cold storage. Cold storage increased PUMP expression as detected by Western blots and led to elevated capacities of both PUMP (44%) and CN-resistant AOX (10 times), but not the cytochrome pathway. Since we found that cold storage led to about the same mitochondrial respiration of 40 nmol O2 min(-1) mg(-1) attributable to each of the respective proteins, we conclude that both AOX and PUMP equally contribute to adaptation of potato tubers to cold.
Subject: Adaptation, Physiological
Carbon Dioxide
Carrier Proteins
Cell Respiration
Cold Temperature
Ion Channels
Kinetics
Membrane Proteins
Mitochondrial Proteins
Oxidoreductases
Plant Proteins
Solanum Tuberosum
Temperature
Transcription, Genetic
Up-regulation
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/13678272
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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