Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195490
Type: Artigo de periódico
Title: Longitudinal Differentiation In Melipona Mandacaia (hymenoptera, Meliponini) Chromosomes.
Author: Rocha, M P
Cruz, M P
Fernandes, A
Waldschmidt, A M
Silva-Júnior, J C
Pompolo, S G
Abstract: Melipona mandacaia is a stingless bee endemic to northeast Brasil. We describe the M. mandacaia karyotype using C-banding technique. fluorochrome staining and treatment with restriction enzymes and discuss the position of this species in the context of the phylogeny of the genus. Melipona mandacaia has 2n = 18 (14 SM + 2 M + 2 A). Heterochromatin was detected in the pericentromeric region of pairs 1, 2 and 8 and in the form of small blocks in the remaining pairs. Staining with base-specific fluorochromes showed that this heterochromatin was rich AT (QM and DAPI), except in the region corresponding to the NOR which was rich GC (CMA3) and was cleaved by the HaeIII enzyme. Melipona mandacaia is a member of Group I Melipona. Treatment with DraI/Giemsa discloses a larger number of bands than treatment with DraI/QM. Pre-cleavage with DraI gave rise to a larger number of bands following QM staining; a circumstance evidently due to a removal of the DNA-protein complex that prevented the association of the fluorochrome with AT-rich DNA. The results highlight the complex nature of heterochromatin.
Subject: Animals
Cerebellum
Chromosome Banding
Fluorescent Dyes
Heterochromatin
Hymenoptera
Karyotyping
Metaphase
Nucleolus Organizer Region
Phylogeny
Restriction Mapping
Staining And Labeling
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/12921165
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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