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Type: Artigo de periódico
Title: Accuracy Of The Serological Elisa Test Compared With The Polymerase Chain Reaction For The Diagnosis Of Cytomegalovirus Infection In Pregnancy.
Author: Parmigiani, Silvana Varella
Barini, Ricardo
Costa, Sandra Cecília Botelho
Amaral, Eliana
da Silva, José Carlos Gama
Pinto e Silva, João Luiz de Carvalho
Abstract: The most frequently used methods for detecting antibodies are the indirect immunofluorescence test and the enzymatic immunoassay (ELISA). The polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific DNA fragments is induced from very low concentrations of complex substrates aloowing the detection of very low amounts of viral particles. To assess the accuracy of serological/ELISA tests in comparison with the polymerase chain reaction in maternal blood to diagnose cytomegalovirus infection. A descriptive study was performed. High-risk outpatient clinic of Campinas University (Unicamp). We selected 243 pregnant women. All of them had been indicated for blood sampling because of suspicions of cytomegalovirus infection and also because of other infections. The group was tested for cytomegalovirus. Serological tests were run and compared to the polymerase chain reaction, which was considered to be the gold standard. Status analyses were done using Fisher's exact test, via the SAS software. The previous cytomegalovirus infection rate was 94.6%. The main reasons for inclusion in the study were fetal nervous system malformation (25.5%), maternal toxoplasmosis (25.5%) and Rh isoimmunization (14.8%). Only two women were included because of positive serological immunoglobulin M test for cytomegalovirus. The sensitivity and specificity of the serological tests were 94% and 6% for immunoglobulin G. Serological tests had lower sensitivity in comparison with the polymerase chain reaction test when diagnosing cytomegalovirus infection. The consequences of positive polymerase chain reaction and negative immunoglobulin M in women remain unknown.
Subject: Adult
Cytomegalovirus Infections
Enzyme-linked Immunosorbent Assay
Immunoglobulin G
Immunoglobulin M
Polymerase Chain Reaction
Pregnancy Complications, Infectious
Serologic Tests
Rights: aberto
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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