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Type: Artigo de periódico
Title: Iron-responsive Genes Of Phanerochaete Chrysosporium Isolated By Differential Display Reverse Transcription Polymerase Chain Reaction.
Author: Assmann, Eliana Maria
Ottoboni, Laura Maria Mariscal
Ferraz, André
Rodríguez, Jaime
De Mello, Maricilda Palandi
Abstract: White-rot fungus Phanerochaete chrysosporium, a ligninolytic basidiomycete, was studied to identify iron-responsive genes. Using the differential display reverse transcription PCR technique (DDRT-PCR), a total of 97 differentially expressed cDNA fragments were identified by comparing band intensities among fingerprints obtained from mycelia cultivated in iron-deficient and iron-replete media. Transcripts induced under iron-starvation exhibited homologies to: a modular polyketide synthase, a TonB protein, a probable transmembrane protein, a putative ABC transporter permease and a HSP70-related heat-shock protein. Modular polyketide synthase and TonB proteins are normally expressed under iron-starvation and are known to be involved in biosynthesis and transport of siderophores respectively. Also, a deduced protein with 96% similarity to a precursor of the well-known P. chrysosporium lignin peroxidase was identified under iron-deficiency. Two DDRT-PCR products confirmed their iron-induced expression. One was homologue to the CNOT3, which is a global regulator of RNA polymerase II transcription and has been implicated in multiple roles in the control of mRNA metabolism. The other was similar to the Schizosaccharomyces pombe putative proteasome maturation factor upm1. In conclusion, the majority of iron-responsive P. chrysosporium transcripts isolated in the DDRT-PCR encode proteins involved in iron acquisition, especially members of biosynthesis and transport of iron chelators.
Subject: Blotting, Northern
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Fungal Proteins
Gene Expression Profiling
Gene Expression Regulation, Fungal
Genes, Fungal
Hsp70 Heat-shock Proteins
Iron-regulatory Proteins
Membrane Proteins
Membrane Transport Proteins
Molecular Sequence Data
Multienzyme Complexes
Rna, Fungal
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, Dna
Sequence Homology, Nucleic Acid
Transcription, Genetic
Citation: Environmental Microbiology. v. 5, n. 9, p. 777-86, 2003-Sep.
Rights: fechado
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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