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Type: Artigo de periódico
Title: Alpha-cardiac Actin (actc) Binds To The Band 3 (ae1) Cardiac Isoform.
Author: Moura Lima, Paulo Roberto
Salles, Teresa Sueko Ide
Costa, Fernando Ferreira
Saad, Sara Teresinha Olalla
Abstract: The band 3 protein is the major integral protein present in the erythrocyte membrane. Two tissue-specific isoforms are also expressed in kidney alpha intercalated cells and in cardiomyocytes. It has been suggested that the cardiac isoform predominantly mediates the anion exchange in cardiomyocytes, but the role of the cytoplasmic domain of the band 3 (CDB3) protein in the cardiac tissue is unknown. In order to characterize novel associations of the CDB3 in the cardiac tissue, we performed the two-hybrid assay, using a bait comprising the region from leu 258 to leu 311 of the erythrocyte band 3, which must also be present in the cardiac isoform. The assay revealed two clones containing the C-terminal region of the alpha-cardiac actin. Immunoprecipitation of whole rat heart using an anti-actin antibody, immunoblotted with anti-human band 3, showed that actin binds to band 3 which was confirmed in the reverse assay. The confocal microscopy showed band 3 in the intercalated discs. Thus, besides the in vivo physical interaction in the Saccharomyces cerevisiae cell, we demonstrated using immunopreciptation that there is a physical association of band 3 with alpha-cardiac actin in cardiomyocyte, and we suggest that the binding occur in situ, in the intercalated disc, a site of cell-cell contact and attachment of the sarcomere to the plasma membrane.
Subject: Actins
Amino Acid Sequence
Anion Exchange Protein 1, Erythrocyte
Microscopy, Confocal
Molecular Sequence Data
Polymerase Chain Reaction
Precipitin Tests
Protein Isoforms
Saccharomyces Cerevisiae
Two-hybrid System Techniques
Rights: fechado
Identifier DOI: 10.1002/jcb.10561
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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