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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleStructural And Biological Characterization Of A Crotapotin Isoform Isolated From Crotalus Durissus Cascavella Venom.pt_BR
dc.contributor.authorde Oliveira, Daniela Gpt_BR
dc.contributor.authorToyama, Marcos Hpt_BR
dc.contributor.authorMartins, Alice M Cpt_BR
dc.contributor.authorHavt, Alexandrept_BR
dc.contributor.authorNobre, Arlândia C Lpt_BR
dc.contributor.authorMarangoni, Sergiopt_BR
dc.contributor.authorCâmara, Paula Rpt_BR
dc.contributor.authorAntunes, Edsonpt_BR
dc.contributor.authorde Nucci, Gilbertopt_BR
dc.contributor.authorBeliam, Luis O Spt_BR
dc.contributor.authorFonteles, Manassés Cpt_BR
dc.contributor.authorMonteiro, Helena S Apt_BR
unicamp.authorDaniela G de Oliveira, Department of Biochemistry, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.pt_BR
unicamp.author.externalMarcos H Toyama,pt
unicamp.author.externalAlice M C Martins,pt
unicamp.author.externalAlexandre Havt,pt
unicamp.author.externalArlândia C L Nobre,pt
unicamp.author.externalSergio Marangoni,pt
unicamp.author.externalPaula R Câmara,pt
unicamp.author.externalEdson Antunes,pt
unicamp.author.externalGilberto de Nucci,pt
unicamp.author.externalLuis O S Beliam,pt
unicamp.author.externalManassés C Fonteles,pt
unicamp.author.externalHelena S A Monteiro,pt
dc.subjectActinomycetalespt_BR
dc.subjectAmino Acid Sequencept_BR
dc.subjectAnimalspt_BR
dc.subjectChromatography, High Pressure Liquidpt_BR
dc.subjectCrotalid Venomspt_BR
dc.subjectCrotoxinpt_BR
dc.subjectDose-response Relationship, Drugpt_BR
dc.subjectDrug Synergismpt_BR
dc.subjectEdemapt_BR
dc.subjectHindlimbpt_BR
dc.subjectKidneypt_BR
dc.subjectMalept_BR
dc.subjectMolecular Sequence Datapt_BR
dc.subjectPerfusionpt_BR
dc.subjectPhospholipases Apt_BR
dc.subjectPhospholipases A2pt_BR
dc.subjectProtein Isoformspt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectSequence Alignmentpt_BR
dc.subjectSkinpt_BR
dc.subjectXanthomonaspt_BR
dc.description.abstractEnvenoming by Crotalus durissus subspecies leads to coagulation disorders, myotoxicity, neurotoxicity and acute renal failure. The most serious systemic alteration and primary cause of death after snakebite is acute renal failure. In this work, we isolated crotapotin, an acid component (Crtp) of crotoxin from Crotalus durissus cascavella venom and we investigated its bactericidal and pro-inflammatory activities as well as its renal effects in rat isolated perfused kidneys. Crtp was bactericidal to the Gram-negative species Xanthomonas axonopodis pv. passiflorae, but was less effective against the Gram-positive Claribacteri ssp, probably because of differences in the cell wall composition. Crtp showed a high amino acid sequence homology with other Crtps described in the literature (around of 90%) and its A and B chains had high conserved regions corresponding to the calcium-binding loop, catalytic site and helix 3 of PLA2. The Crtp showed moderate pro-inflammatory activity and increased significantly the inflammation evoked by PLA2 when co-injected or co-incubated with PLA2. The renal parameters evaluated included the perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR) and percent of sodium tubular transport (%TNa+). Crotapotin (5 microg/ml) significantly increased the PP and RVR, whereas the GFR, UF and %TNa+ were unaffected. These results suggest that crotoxin is the main venom component responsible for nephrotoxicity and crotapotin contributes little to this phenomenom. The biological and bactericidal actions of Crtp also suggest that this protein may have functions other than simply acting as a chaperone for PLA2.en
dc.relation.ispartofToxicon : Official Journal Of The International Society On Toxinologypt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.date.issued2003-Julpt_BR
dc.identifier.citationToxicon : Official Journal Of The International Society On Toxinology. v. 42, n. 1, p. 53-62, 2003-Jul.pt_BR
dc.language.isoengpt_BR
dc.description.volume42pt_BR
dc.description.firstpage53-62pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12893061pt_BR
dc.date.available2015-11-27T12:52:20Z-
dc.date.accessioned2015-11-27T12:52:20Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:52:20Z (GMT). No. of bitstreams: 1 pmed_12893061.pdf: 498632 bytes, checksum: 5e341f9267f5939a655c03ce90cfd4a0 (MD5) Previous issue date: 2003en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195475-
dc.identifier.idPubmed12893061pt_BR
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