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Type: Artigo de periódico
Title: Influence Of Protein Phosphatase Inhibitors On Hl60 Cells Death Induction By Dehydrocrotonin.
Author: Freire, Ana Claudia Galvão
de Assis, Cristiane Fernandes
Frick, Alexander Otto
da Silva Melo, Patrícia
Haun, Marcela
Aoyama, Hiroshi
Durán, Nelson
Sauer, Mariana Melillo
Kallás, Esper Georges
Ferreira, Carmen Veríssima
Abstract: Oxidative stress can be involved in several cellular responses, such as differentiation, apoptosis and necrosis. Dehydrocrotonin (DCTN, diterpene lactone) from Croton cajucara, Brazilian medicinal plant, slightly induced NBT-reducing activity. In presence of protein phosphatase inhibitors significant differentiation of HL60 cells was observed. Flow cytometry analysis demonstrated that apoptosis was induced when the cells were treated with okadaic acid (OKA) and plus trans-dehydrocrotonin (t-DCTN) this effect was two-fold increased. Unlike, when the cells were treated only with t-DCTN, necrosis was observed. On the other hand, the necrosis induced by t-DCTN could be due to oxidative stress, revealed by increase of GSH content. Therefore, this differentiation pathway involves the modulation of protein phosphatases and this inhibition promotes the t-DCTN action on apoptosis induction.
Subject: Annexin A5
Cell Differentiation
Diterpenes, Clerodane
Drug Screening Assays, Antitumor
Enzyme Inhibitors
Flow Cytometry
Hl-60 Cells
Leukemia, Promyelocytic, Acute
Okadaic Acid
Oxidative Stress
Phosphoprotein Phosphatases
Plants, Medicinal
Citation: Leukemia Research. v. 27, n. 9, p. 823-9, 2003-Sep.
Rights: fechado
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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