Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Comparative Cytotoxicity Of Dimethylamide-crotonin In The Promyelocytic Leukemia Cell Line (hl60) And Human Peripheral Blood Mononuclear Cells.
Author: Anazetti, Maristella Conte
Melo, Patricia Silva
Durán, Nelson
Haun, Marcela
Abstract: Dehydrocrotonin (DHC) is a diterpene lactone obtained from Croton cajucara (Sacaca). Dimethylamide-crotonin (DCR), a DHC derivative, has a similar inhibitory effect on leukemic HL60 cells than its parent compound evaluated by different endpoints of cytotoxicity. No cytotoxicity or morphological alterations associated with apoptosis were detected in human peripheral blood mononuclear cells (PBMC) after treatment with up to 400 micro M DCR in presence of phytohemaglutinin (5 micro g/ml). Based on morphological changes and the pattern of DNA fragmentation, DHC and DCR were found to induce apoptosis and terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells, but these compounds did not show any toxic effect in PBMC. Thus, DCR and DHC inhibit HL60 cell growth in vitro partly by inducing apoptosis and cell differentiation, but does not cause serious damage to immune cells according to our experimental conditions.
Subject: Antineoplastic Agents, Phytogenic
Cell Differentiation
Cell Division
Dna Fragmentation
Diterpenes, Clerodane
Electrophoresis, Agar Gel
Hl-60 Cells
Leukocytes, Mononuclear
Nitroblue Tetrazolium
Plants, Medicinal
Citation: Toxicology. v. 188, n. 2-3, p. 261-74, 2003-Jun.
Rights: fechado
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_12767696.pdf627.56 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.