Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195393
Type: Artigo de periódico
Title: Warifteine And Milonine, Alkaloids Isolated From Cissampelos Sympodialis Eichl: Cytotoxicity On Rat Hepatocyte Culture And In V79 Cells.
Author: Melo, Patricia Silva
de Medeiros Cavalcante, Horacinna Maria
Barbosa-Filho, José Maria
de Fátima Formiga Melo Diniz, Margareth
de Medeiros, Isac Almeida
Haun, Marcela
Abstract: Two alkaloids were isolated from the leaves of Cissampelos sympodialis; a bisbenzylisoquinoline compound named warifteine and a novel 8,14-dihydromorphinandienone alkaloid named milonine. The cytotoxic effects of these alkaloids were assayed in cultured hepatocytes and V79 fibroblasts. Three independent endpoint assays for cytotoxicity in vitro were used: the nucleic acid content (NAC), tetrazolium reduction (MTT) and neutral red uptake (NRU). Milonine was less toxic than warifteine in both cell cultures. The IC50 values determined in the three different viability assays were around 100 and 400 microM after milonine treatment of V79 cells or hepatocytes. IC50 values ranging from 10 to 35 microM were obtained for warifteine in the viability tests evaluated in V79 cells and hepatocytes. Due to the similar cytotoxic effects detected on V79 cells and hepatocytes, probably warifteine and milonine induced toxic effects independent to the cytochrome P450. This hypothesis was corroborated by the results where Cimetidine (1.0 mM), a traditional cytochrome P450 inhibitor, did not protect the cells from the toxic action of warifteine or milonine. In conclusion, these alkaloids merit further investigations as potential novel pharmacological agents although milonine was less toxic than warifteine in the cells models investigated.
Subject: Alkaloids
Animals
Cell Survival
Cimetidine
Cissampelos
Coloring Agents
Cricetinae
Cricetulus
Cytochrome P-450 Enzyme Inhibitors
Cytochrome P-450 Enzyme System
Enzyme Inhibitors
Fibroblasts
Formazans
Hepatocytes
Male
Neutral Red
Plant Roots
Rats
Rats, Wistar
Tetrazolium Salts
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/12765248
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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