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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleGenotyping Of Human Cytomegalovirus Using Non-radioactive Single-strand Conformation Polymorphism (sscp) Analysis.pt_BR
dc.contributor.authorde Albuquerque, Dulcinéia Martinspt_BR
dc.contributor.authorCosta, Sandra Cecília Botelhopt_BR
unicamp.authorDulcinéia Martins de Albuquerque, Department of Pharmacology, College of Medicine, State University of Campinas, Distrito de Barão Geraldo, SP 13083-970, Campinas, Brazil. dulmal@unicamp.br <dulmal@unicamp.br>pt_BR
unicamp.author.externalSandra Cecília Botelho Costa,pt
dc.subjectBone Marrow Transplantationpt_BR
dc.subjectCytomegaloviruspt_BR
dc.subjectCytomegalovirus Infectionspt_BR
dc.subjectGenetic Variationpt_BR
dc.subjectGenotypept_BR
dc.subjectHumanspt_BR
dc.subjectPoint Mutationpt_BR
dc.subjectPolymerase Chain Reactionpt_BR
dc.subjectPolymorphism, Restriction Fragment Lengthpt_BR
dc.subjectPolymorphism, Single-stranded Conformationalpt_BR
dc.subjectReproducibility Of Resultspt_BR
dc.subjectSensitivity And Specificitypt_BR
dc.subjectViral Envelope Proteinspt_BR
dc.description.abstractGenetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs) pathogenesis. Using restriction analysis of the gB gene product (PCR-RFLP), amplified by the nested polymerase chain reaction, the HCMV strains can be compared and classified into at least four HCMV groups. PCR single-strand conformation polymorphism (PCR-SSCP) is one of the techniques used to identify a mutant sequence or a polymorphism in a known gene. SSCP analysis has the advantage over RFLP analysis on detection of DNA polymorphisms and point mutations at a variety of positions in DNA fragments. However, the original SSCP protocols using the incorporation of radioactive label and polyacrylamide gel electrophoresis for detection are labour intensive and time-consuming. A simplified SSCP protocol is described to identify HCMV strains and the gB genotype, allowing the detection of sequence variations not residing in the endonuclease recognition sites.en
dc.relation.ispartofJournal Of Virological Methodspt_BR
dc.relation.ispartofabbreviationJ. Virol. Methodspt_BR
dc.date.issued2003-Junpt_BR
dc.identifier.citationJournal Of Virological Methods. v. 110, n. 1, p. 25-8, 2003-Jun.pt_BR
dc.language.isoengpt_BR
dc.description.volume110pt_BR
dc.description.firstpage25-8pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0166-0934pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12757917pt_BR
dc.date.available2015-11-27T12:52:08Z-
dc.date.accessioned2015-11-27T12:52:08Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:52:08Z (GMT). No. of bitstreams: 1 pmed_12757917.pdf: 220504 bytes, checksum: 3916796185d74080967de842a90db80c (MD5) Previous issue date: 2003en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195388-
dc.identifier.idPubmed12757917pt_BR
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