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Type: Artigo de periódico
Title: Bcr-abl Binds To Irs-1 And Irs-1 Phosphorylation Is Inhibited By Imatinib In K562 Cells.
Author: Traina, Fabíola
Carvalheira, José Barreto C
Saad, Mario J A
Costa, Fernando F
Saad, Sara T O
Abstract: In the present study we used K562 cells to demonstrate that insulin receptor substrate 1 (IRS-1) is expressed and constitutively phosphorylated in BCR-ABL(+) cells. We observed association between BCR-ABL/IRS-1, IRS-1/phosphoinositide 3'-kinase (PI3-kinase), and IRS-1/Grb2 in the K562 cell line. Our findings demonstrate that imatinib treatment resulted in marked attenuation of BCR-ABL/IRS-1 association and of IRS-1-stimulated PI3-kinase activity in K562 cells. We concluded that the IRS-1 protein is involved in the signalling pathway of the BCR-ABL tyrosine kinase.
Subject: Adaptor Proteins, Signal Transducing
Blotting, Western
Dose-response Relationship, Drug
Enzyme Activation
Enzyme Inhibitors
Fusion Proteins, Bcr-abl
Grb2 Adaptor Protein
Hl-60 Cells
Insulin Receptor Substrate Proteins
K562 Cells
Phosphatidylinositol 3-kinases
Protein Binding
Rights: fechado
Identifier DOI: 
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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