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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleProteins Induced By Xanthomonas Axonopodis Pv. Passiflorae With Leaf Extract Of The Host Plant (passiflorae Edulis).pt_BR
dc.contributor.authorTahara, Sandra Tpt_BR
dc.contributor.authorMehta, Angelapt_BR
dc.contributor.authorRosato, Yoko Bpt_BR
unicamp.authorSandra T Tahara, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, Brazil.pt_BR
unicamp.author.externalAngela Mehta,pt
unicamp.author.externalYoko B Rosato,pt
dc.subjectAmino Acid Sequencept_BR
dc.subjectBacterial Proteinspt_BR
dc.subjectElectrophoresis, Gel, Two-dimensionalpt_BR
dc.subjectKineticspt_BR
dc.subjectPassiflorapt_BR
dc.subjectPeptide Mappingpt_BR
dc.subjectPlant Extractspt_BR
dc.subjectPlant Leavespt_BR
dc.subjectProteomept_BR
dc.subjectXanthomonaspt_BR
dc.description.abstractTwo-dimensional gel electrophoresis was used to identify differentially displayed proteins during treatment of Xanthomonas axonopodis pv. passiflorae in media containing leaf extract of the compatible (passion fruit) and incompatible (tomato) hosts. The results showed that at different times of treatment (5, 25 and 45 h) the global expression of proteins was almost identical in cells grown in minimal medium (MM) and in medium containing leaf extract of the incompatible host (MMT). The protein patterns of cells grown in medium containing passiflorae (MMP) leaf extract and MM were also compared enabling the detection of 17 differential spots. Most of the proteins were induced at earlier times of incubation (5 h) and maintained until 45 h in MMP. By using another carrier ampholyte range, seven additional proteins were identified in MMP treated cells. Five proteins, including one constitutive, two induced and two up-regulated in MMP were microsequenced. All sequences were found in the genome of xanthomonads sharing high level of identity (88-100%). Fructose biphosphate aldolase was expressed in all media employed. A putative membrane-related protein and a hypothetical protein were novel proteins induced specifically by the passiflorae extract. An inorganic pyrophosphatase and a hypothetical protein that showed similarity to the yciF gene of Salmonella thyphimurium were up-regulated in MMP.en
dc.relation.ispartofProteomicspt_BR
dc.relation.ispartofabbreviationProteomicspt_BR
dc.date.issued2003-Janpt_BR
dc.identifier.citationProteomics. v. 3, n. 1, p. 95-102, 2003-Jan.pt_BR
dc.language.isoengpt_BR
dc.description.volume3pt_BR
dc.description.firstpage95-102pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1615-9853pt_BR
dc.identifier.doi10.1002/pmic.200390014pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12548639pt_BR
dc.date.available2015-11-27T12:52:04Z-
dc.date.accessioned2015-11-27T12:52:04Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:52:04Z (GMT). No. of bitstreams: 1 pmed_12548639.pdf: 238043 bytes, checksum: d54ab9271d11470f54ff5e481890e606 (MD5) Previous issue date: 2003en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195361-
dc.identifier.idPubmed12548639pt_BR
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