Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195330
Type: Artigo de periódico
Title: A Technique For Mycelial Development Of Ectomycorrhizal Fungi On Agar Media.
Author: De Araújo, Alvaro Alberto
Roussos, Sevastianos
Abstract: A technique was established to study ectomycorrhizal fungi on agar media. Petri dishes, 60 mm in diameter, containing 10 mL of culture medium covered with a cellophane disk were used for easy collection of the mycelium after growth. For analysis of fungal biomass production, a sterilized cellophane sheet was placed on the medium's surface. Inoculation was achieved by placing a mycelial block onto the center of the cellophane sheet and then incubating at 25 degrees C in the dark. Colony radial growth was measured and biomass dry wt was determined. Fresh mycelia were homogenized with 10 mL of acetate buffer (pH 5.5) for enzyme analysis. A crude extract was obtained by adding all culture medium to 90 mL of distilled water and homogenizing in a Potter. Reducing sugars, enzyme concentration, and pH were determined. Three fungal strains, Suillus collinitus, Pisosithus arrhizus, and Hebeloma cylindrosporum, were grown in different culture media (potato dextrose agar or Pintro's medium). Parameters measured over time included glucose concentration, phosphatase activity, biomass, and pH.
Subject: Agar
Biomass
Culture Media
Glucose
Hydrogen-ion Concentration
Mycology
Mycorrhizae
Phosphoric Monoester Hydrolases
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/12018258
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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