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Type: Artigo de periódico
Title: Correlation Of Mixed Lymphocyte Culture With Chronic Graft-versus-host Disease Following Allogeneic Stem Cell Transplantation.
Author: Visentainer, J E L
Lieber, S R
Persoli, L B L
de Souza Lima, S C B
Vigorito, A C
Aranha, F J P
Eid, K A B
Oliveira, G B
Miranda, E C M
de Souza, C A
Abstract: The purpose of the present study was to evaluate the mixed lymphocyte culture as a predictive assay of acute and chronic graft-versus-host disease (GVHD). We studied 153 patients who received a first bone marrow transplantation from human leukocyte antigen-identical siblings. Acute GVHD was observed in 26 of 128 (20.3%) patients evaluated and chronic GVHD occurred in 60 of 114 (52.6%). One-way mixed lymphocyte culture (MLC) assays were performed by the standard method. MLC results are reported as the relative response (RR) from donor against patient cells. The responses ranged from -47.0 to 40.7%, with a median of 0.5%. The Kaplan-Meier probability of developing GVHD was determined for patients with positive and negative MLC. There was no significant difference in incidence of acute GVHD between the groups studied. However, the incidence of chronic GVHD was higher in recipients with RR >4.5% than in those with RR < or =4.5%. The Cox Proportional Hazards model was used to examine the effect of MLC levels on incidence of chronic GVHD, while adjusting for the potential confounding effect of others suspected or observed risk factors. The relative risk of chronic GVHD was 2.5 for patients with positive MLC (RR >4.5%), 2.9 for those who received peripheral blood progenitor cells as a graft, and 2.2 for patients who developed previous acute GVHD. MLC was not useful for predicting acute GVHD, but MLC with RR >4.5% associated with other risk factors could predict the development of chronic GVHD, being of help for the prevention and/or treatment of this late complication.
Subject: Acute Disease
Child, Preschool
Chronic Disease
Graft Vs Host Disease
Hla Antigens
Hematopoietic Stem Cell Transplantation
Lymphocyte Culture Test, Mixed
Middle Aged
Predictive Value Of Tests
Risk Factors
Transplantation, Homologous
Citation: Brazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 35, n. 5, p. 567-72, 2002-May.
Rights: aberto
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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