Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195288
Type: Artigo de periódico
Title: Iron-regulated Proteins In Phanerochaete Chrysosporium And Lentinula Edodes: Differential Analysis By Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis And Two-dimensional Polyacrylamide Gel Electrophoresis Profiles.
Author: Hernández-Macedo, Maria L
Ferraz, André
Rodríguez, Jaime
Ottoboni, Laura M M
De Mello, Maricilda Palandi
Abstract: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE) were used to identify iron-responsive proteins in the white-rot species (Phanerochaete chrysosporium and Lentinula edodes), by comparing the differential patterns of cellular and membrane proteins obtained from iron-sufficient and iron-deficient mycelia. Six cellular proteins induced by iron restriction have been observed in SDS-PAGE for P. chrysosporium and twelve for L. edodes. In 2-DE, the numbers of iron-restricted induced proteins were 12 and 9, respectively, in a resolution range of 15-60 kDa and pI 4.5-8.1. SDS-PAGE for the plasma membrane protein did not show differences, whereas the outer-membrane protein profile showed 6 and 5 proteins induced by iron depletion in P. chrysosporium and L. edodes, respectively. The results presented here are important data to unravel mechanisms of biosynthesis and/or transport of the iron-complexing agents in ligninolytic fungi and to further correlate them to the ligninolytic processes.
Subject: Chrysosporium
Electrophoresis, Gel, Two-dimensional
Electrophoresis, Polyacrylamide Gel
Fungal Proteins
Gene Expression Regulation, Fungal
Iron
Shiitake Mushrooms
Citation: Electrophoresis. v. 23, n. 4, p. 655-61, 2002-Feb.
Rights: fechado
Identifier DOI: 10.1002/1522-2683(200202)23:4<655::AID-ELPS655>3.0.CO;2-S
Address: http://www.ncbi.nlm.nih.gov/pubmed/11870778
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_11870778.pdf3.66 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.