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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDifferential Gene Expression In Response To Copper In Acidithiobacillus Ferrooxidans Analyzed By Rna Arbitrarily Primed Polymerase Chain Reaction.pt_BR
dc.contributor.authorPaulino, Luciana Campospt_BR
dc.contributor.authorde Mello, Maricilda Ppt_BR
dc.contributor.authorOttoboni, Laura M Mpt_BR
unicamp.authorLuciana Campos Paulino, Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil.pt_BR
unicamp.author.externalMaricilda P de Mello,pt
unicamp.author.externalLaura M M Ottoboni,pt
dc.subjectBase Sequencept_BR
dc.subjectCopperpt_BR
dc.subjectGammaproteobacteriapt_BR
dc.subjectGene Expression Profilingpt_BR
dc.subjectGene Expression Regulation, Bacterialpt_BR
dc.subjectPolymerase Chain Reactionpt_BR
dc.subjectRna, Bacterialpt_BR
dc.subjectThiobacilluspt_BR
dc.description.abstractAcidithiobacillus ferrooxidans is a chemoautotrophic bacterium that plays an important role in metal bioleaching processes. Despite the high level of tolerance to heavy metals shown by A. ferrooxidans, the genetic basis of copper resistance in this species remains unknown. We investigated the gene expression in response to copper in A. ferrooxidans LR using RNA arbitrarily primed polymerase chain reaction (RAP-PCR). One hundred and four differentially expressed genes were identified using eight arbitrary primers. Differential gene expression was confirmed by DNA slot blot hybridization, and approximately 70% of the RAP-PCR products were positive. The RAP-PCR products that presented the highest levels of induction or repression were cloned, sequenced and the sequences were compared with those in databases using the BLAST search algorithm. Seventeen sequences were obtained. The RAP-PCR product with the highest induction ratio showed similarity with the A. ferrooxidans cytochrome c. A high similarity with the thiamin biosynthesis gene thiC from Caulobacter crescentus was observed for another RAP-PCR product induced by copper. An RAP-PCR product repressed by copper showed significant similarity with the carboxysome operon that includes the ribulose-1,5-bisphosphate carboxylase/oxygenase complex from A. ferrooxidans and another copper-repressed product was significantly similar to the XyIN outer membrane protein from Pseudomonas putida. Finally, RAP-PCR products of unknown similarities were also present.en
dc.relation.ispartofElectrophoresispt_BR
dc.relation.ispartofabbreviationElectrophoresispt_BR
dc.date.issued2002-Febpt_BR
dc.identifier.citationElectrophoresis. v. 23, n. 4, p. 520-7, 2002-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume23pt_BR
dc.description.firstpage520-7pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0173-0835pt_BR
dc.identifier.doi10.1002/1522-2683(200202)23:4<520::AID-ELPS520>3.0.CO;2-Rpt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/11870759pt_BR
dc.date.available2015-11-27T12:49:33Z-
dc.date.accessioned2015-11-27T12:49:33Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:49:33Z (GMT). No. of bitstreams: 1 pmed_11870759.pdf: 1390956 bytes, checksum: 665cc5aa11d18a12f61aa1a821120147 (MD5) Previous issue date: 2002en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195287-
dc.identifier.idPubmed11870759pt_BR
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