Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195184
Type: Artigo de periódico
Title: Kinetics Of Tnf-alpha And Ifn-gamma Mrna Expression In Islets And Spleen Of Nod Mice.
Author: Ventura-Oliveira, D
Vilella, C A
Zanin, M E
Castro, G M
Moreira Filho, D C
Zollner, R L
Abstract: Insulin-dependent diabetes mellitus is caused by autoimmune destruction of pancreatic beta cells. Non-obese diabetic (NOD) mice spontaneously develop diabetes similar to the human disease. Cytokines produced by islet-infiltrating mononuclear cells may be directly cytotoxic and can be involved in islet destruction coordinated by CD4+ and CD8+ cells. We utilized a semiquantitative RT-PCR assay to analyze in vitro the mRNA expression of TNF-alpha and IFN-gamma cytokine genes in isolated islets (N = 100) and spleen cells (5 x 10(5) cells) from female NOD mice during the development of diabetes and from female CBA-j mice as a related control strain that does not develop diabetes. Cytokine mRNAs were measured at 2, 4, 8, 14 and 28 weeks of age from the onset of insulitis to the development of overt diabetes. An increase in IFN-gamma expression in islets was observed for females aged 28 weeks (149 +/- 29 arbitrary units (AU), P<0.05, Student t-test) with advanced destructive insulitis when compared with CBA-j mice, while TNF-alpha was expressed in both NOD and CBA-j female islets at the same level at all ages studied. In contrast, TNF-alpha in spleen was expressed at higher levels in NOD females at 14 weeks (99 +/- 8 AU, P<0.05) and 28 weeks (144 +/- 17 AU, P<0.05) of age when compared to CBA-j mice. The data suggest that IFN-gamma and TNF-alpha expression in pancreatic islets of female NOD mice is associated with beta cell destruction and overt diabetes.
Subject: Age Factors
Animals
Diabetes Mellitus, Type 1
Female
Gene Expression
Interferon-gamma
Islets Of Langerhans
Kinetics
Mice
Mice, Inbred Cba
Mice, Inbred Nod
Rna, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Spleen
Tumor Necrosis Factor-alpha
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/12426635
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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