Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195134
Type: Artigo de periódico
Title: Neuromuscular Action Of Bothrops Lanceolatus (fer De Lance) Venom And A Caseinolytic Fraction.
Author: Lôbo de Araújo, Albetiza
Donato, José Luiz
Leite, Gildo Bernardo
Prado-Franceschi, Júlia
Fontana, Marcos Dias
Bon, Cassian
Rodrigues Simioni, Léa
Abstract: A protein capable of inducing neuromuscular blockade in avian preparations and of depolarizing mouse diaphragm muscle was isolated from Bothrops lanceolatus venom using gel filtration and ion-exchange chromatography. The purified protein was a single chain polypeptide with an estimated molecular mass of 27.5 kDa by SDS-PAGE and had caseinolytic activity (13.3 units/mg), but no phospholipase A(2). B.lanceolatus venom (50 micro g/ml) and the caseinolytic protein (20 micro g/ml) produced contracture and progressive irreversible blockade (50% in 25+/-5 min (SEM) and 45+/-15 min, respectively), in indirectly stimulated chick biventer cervicis preparations. The contractile responses to acetylcholine (ACh; 37 and 74 micro M, n=6) were inhibited by venom and the caseinolytic protein, whereas those to potassium (13.4mM, n=6) were not. Membrane resting potential measurements in mouse hemidiaphragm preparations showed that B.lanceolatus venom and the purified protein caused depolarization which was prevented by D-tubocurarine (14.6mM). The venom produced a slight increase in the amplitude and frequency of miniature end-plate potentials, but this effect was not seen with the purified fraction. These results suggest that the purified protein acts exclusively post-synaptically.
Subject: Animals
Bothrops
Caseins
Chickens
Chromatography, Gel
Chromatography, Ion Exchange
Crotalid Venoms
Diaphragm
Electrophoresis, Polyacrylamide Gel
Male
Membrane Potentials
Mice
Motor Endplate
Muscle Contraction
Neuromuscular Blockade
Neuromuscular Blocking Agents
Neuromuscular Junction
Peptide Hydrolases
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/12220713
Date Issue: 2002
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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