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Type: Artigo de periódico
Title: Preparative Chromatography Of Xylanase Using Expanded Bed Adsorption.
Author: Silvino, DosSantosEveraldo
Guirardello, Reginaldo
Teixeira, Franco Telma
Abstract: Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification.
Subject: Adsorption
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Xylan Endo-1,3-beta-xylosidase
Citation: Journal Of Chromatography. A. v. 944, n. 1-2, p. 217-24, 2002-Jan.
Rights: fechado
Identifier DOI: 
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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