Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195109
Type: Artigo de periódico
Title: Transcription Of Succinate Dehydrogenase Subunit 4 (sdh4) Gene In Potato: Detection Of Extensive Rna Editing And Co-transcription With Cytochrome Oxidase Subunit Iii (cox3) Gene.
Author: Siqueira, Susely F
Dias, Sandra Martha G
Hardouin, Patrice
Pereira, Fabrício R S
Lejeune, Bernard
de Souza, Anete P
Abstract: The potato (Solanum tuberosum L.) mitochondrial cox3/ sdh4 gene cluster was previously identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In this work, this potato gene cluster was cloned, sequenced and its expression was evaluated. The gene sequence and gene locus organization were found to be similar to the corresponding gene cluster in other dicot species, where known. Northern hybridizations with potato and cauliflower (Brassica oleraceae) mtRNA and RT-PCR analyses using potato mtRNA indicated that cox3 and sdh4 are co-transcribed in both species, generating a complex transcription pattern, where several transcripts from 1.1 kb to 4.4 kb are found. The potato transcript from this cluster displayed 14 and 13 RNA-editing sites, in the cox3 and sdh4 genes respectively, which changed the codon identity to amino acids and created a sdh4 partially edited stop codon. Forty-three cDNA clones were analyzed for editing process and revealed different partial-editing with no apparent sequential processing in the sdh4 gene.
Subject: Arabidopsis Proteins
Base Sequence
Conserved Sequence
Dna, Mitochondrial
Electron Transport Complex Iv
Gene Expression
Genes, Plant
Membrane Proteins
Molecular Sequence Data
Plant Proteins
Rna
Rna Editing
Sequence Homology, Nucleic Acid
Solanum Tuberosum
Transcription, Genetic
Rights: fechado
Identifier DOI: 10.1007/s00294-002-0294-y
Address: http://www.ncbi.nlm.nih.gov/pubmed/12172970
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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