Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195102
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDerivatives Of Dehydrocrotonin, A Diterpene Lactone Isolated From Croton Cajucara: Cytotoxicity In Rat Cultured Hepatocytes And In V79 Cells.pt_BR
dc.contributor.authorMelo, P Spt_BR
dc.contributor.authorDurán, Npt_BR
dc.contributor.authorHaun, Mpt_BR
unicamp.authorP S Melo, Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil. pmelo@unicamp.brpt_BR
unicamp.author.externalN Durán,pt
unicamp.author.externalM Haun,pt
dc.subjectAnimalspt_BR
dc.subjectCell Agingpt_BR
dc.subjectCell Linept_BR
dc.subjectCell Survivalpt_BR
dc.subjectCimetidinept_BR
dc.subjectCricetinaept_BR
dc.subjectCricetuluspt_BR
dc.subjectCrotonpt_BR
dc.subjectCulture Media, Conditionedpt_BR
dc.subjectDnapt_BR
dc.subjectDiterpenespt_BR
dc.subjectDiterpenes, Clerodanept_BR
dc.subjectDose-response Relationship, Drugpt_BR
dc.subjectFibroblastspt_BR
dc.subjectHepatocytespt_BR
dc.subjectMalept_BR
dc.subjectNeutral Redpt_BR
dc.subjectPlants, Medicinalpt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectSuccinate Dehydrogenasept_BR
dc.subjectTetrazolium Saltspt_BR
dc.subjectThiazolespt_BR
dc.description.abstractDerivatives of dehydrocrotonin (DHC; Compound I) with different anti-ulcerogenic properties but less toxicity were produced by reducing the cyclohexenone moiety of DHC with NaBH4 (Compound II), reducing the cyclohexenone and lactone moieties with LiAlH4 (Compound III) and transforming the lactone moiety into an amide (Compound IV) using dimethylamine. Derivatives of DHC were assayed in cultured hepatocytes and V79 fibroblasts. Three independent endpoints assays for cytotoxicity were used, namely, the DNA content, tetrazolium reduction (MTT) and neutral red uptake (NRU). Compound III was less toxic than the other DHC derivatives in both cell cultures. ICso values ranging from 250 to 600 microM were obtained for Compounds II and IV in the NRU and DNA content tests evaluated in 4-hour hepatocyte cultures. Although Compound II showed relatively low cytotoxicity in rat hepatocytes based on the NRU and DNA content assays, a very high toxicity (IC50=10 microM) was observed in the MTT test. Metabolites of Compound II in conditioned medium from 4-hour old hepatocyte cultures enhanced the MTT-reducing ability of V79 fibroblasts. The cytotoxicity of the derivatives was greater in recently isolated hepatocytes (only a 4-hour incubation for cell attachment prior to treating with the derivatives) than in hepatocytes previously cultured (24-hour incubation) before the treatment. Thus, aging reduced the cytotoxic effects of DHC derivatives in isolated hepatocytes, suggesting that P450-mediated biotransformation of such derivatives may lead to the formation of more toxic metabolites.en
dc.relation.ispartofHuman & Experimental Toxicologypt_BR
dc.relation.ispartofabbreviationHum Exp Toxicolpt_BR
dc.date.issued2002-Maypt_BR
dc.identifier.citationHuman & Experimental Toxicology. v. 21, n. 5, p. 281-8, 2002-May.pt_BR
dc.language.isoengpt_BR
dc.description.volume21pt_BR
dc.description.firstpage281-8pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0960-3271pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12141400pt_BR
dc.date.available2015-11-27T12:49:11Z-
dc.date.accessioned2015-11-27T12:49:11Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:49:11Z (GMT). No. of bitstreams: 1 pmed_12141400.pdf: 279431 bytes, checksum: 676ce4866296939615d0815f934a97fc (MD5) Previous issue date: 2002en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195102-
dc.identifier.idPubmed12141400pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_12141400.pdf272.88 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.