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Type: Artigo de periódico
Title: Regulation Of Mitochondrial Thioredoxin Peroxidase I Expression By Two Different Pathways: One Dependent On Camp And The Other On Heme.
Author: Monteiro, Gisele
Pereira, Gonçalo Amarante Guimarães
Netto, Luis Eduardo Soares
Abstract: Mitochondrial isoform of thioredoxin peroxidase (mTPx I) is an antioxidant protein recently described in Saccharomyces cerevisiae. Here we characterized pathways that lead to mTPx I induction in two situations: growth in media containing low glucose concentrations and treatment with peroxides. The induction of mTPx I by growth on low glucose concentrations was dependent on cAMP and on the transcription factors Msn2p/Msn4p as demonstrated by northern blot experiments using yeast strains with deletion of MSN2 and MSN4 genes and also using a strain permeable to cAMP. mTPx I expression was also induced by peroxides in a time- and dose-dependent manner and varied with the carbon source present in the media. Deletion of HAP1 or inhibition of heme synthesis abolished induction of mTPx I by H(2)O(2) on cells which were grown in media containing glucose, indicating that Hap1p is involved in the regulation of this process. mTPx I was induced by H(2)O(2) on glycerol/ethanol-containing media, but we could not associate any transcription factor with this phenomenon. Finally, mTPx I also induced by t-butyl hydroperoxide in a Hap1p-independent manner. In conclusion, mTPx I expression is under a complex regulatory network, which involves, at least, two signaling pathways: one sensing the carbon source (which is signalized by cAMP) and the other sensing the intracellular redox state (which is signalized by heme).
Subject: Cyclic Amp
Dna-binding Proteins
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Neoplasm Proteins
Oxidative Stress
Rna, Messenger
Saccharomyces Cerevisiae
Saccharomyces Cerevisiae Proteins
Transcription Factors
Citation: Free Radical Biology & Medicine. v. 32, n. 3, p. 278-88, 2002-Feb.
Rights: fechado
Identifier DOI: 
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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