Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195076
Type: Artigo de periódico
Title: G120k-peg, A Human Gh Antagonist, Decreases Gh Signal Transduction In The Liver Of Mice.
Author: Thirone, Ana C P
Carvalho, Carla R O
Saad, Mario J A
Abstract: After receptor binding, growth hormone (GH) induces GH receptors (GHR) dimerization and JAK2 is activated after its association with a dimerized GHR, stimulating the tyrosyl phosphorylation of insulin receptor substrate-1 (IRS-1), IRS-2 and Shc proteins. G120K-PEG, a GH antagonist is produced by a mutation that blocks GH action by preventing the GHR dimerization. This study shows that the inhibitory effect of G120K-PEG was maximal with a GH:G120K-PEG ratio of 1:100, as no increase in JAK2 tyrosyl phosphorylation was observed with this dose of GH. When the dose of GH was increased and with a GH:G120K-PEG ratio of 1:10 some tyrosyl phosphorylation of JAK2 could be observed. Additionally, GH-induced IRS-1, IRS-2 and SHC tyrosyl phosphorylation was inhibited approximately 50% at equimolar concentrations of the antagonist of GH and almost abolished with a GH:G120K-PEG ratio of 1:100. The results clearly show that G120K-PEG inhibits GH signal transduction in mouse liver.
Subject: Adaptor Proteins, Signal Transducing
Adaptor Proteins, Vesicular Transport
Animals
Dimerization
Dose-response Relationship, Drug
Hormone Antagonists
Human Growth Hormone
Humans
Insulin Receptor Substrate Proteins
Intracellular Signaling Peptides And Proteins
Janus Kinase 2
Liver
Male
Mice
Phosphoproteins
Phosphorylation
Phosphotyrosine
Polyethylene Glycols
Prolactin
Protein Processing, Post-translational
Protein-tyrosine Kinases
Proteins
Proto-oncogene Proteins
Receptors, Somatotropin
Shc Signaling Adaptor Proteins
Signal Transduction
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/12088868
Date Issue: 2002
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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