Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195042
Type: Artigo de periódico
Title: Regulation Of Insulin-stimulated Tyrosine Phosphorylation Of Shc And Shc/grb2 Association In Liver, Muscle, And Adipose Tissue Of Epinephrine- And Streptozotocin-treated Rats.
Author: Páez-Espinosa, V
Rocha, E M
Velloso, L A
Saad, M J
Abstract: Shc protein phosphorylation has been extensively characterized as the initial step that activates a complex mitogenic pathway through its association with Grb2. In the present study, we investigated the adrenergic control of insulin-induced Shc phosphorylation and Shc-Grb2 association, and the modulating effect of streptozotocin-induced diabetes mellitus on Shc phosphorylation and Shc/Grb2 association. Acute treatment with epinephrine, which leads to a normoglycemic insulin-resistant state, does not affect insulin-induced Shc tyrosine phosphorylation or Shc-Grb2 association in liver, muscle, or fat. By contrast, a significant increase in insulin-induced Shc phosphorylation is observed in liver and muscle of rats treated with streptozotocin. The association of Shc/Grb2 is also increased in both tissues following insulin treatment. These data suggest that while epinephrine preserves the insulin-induced phosphorylation of Shc and the mitogenic pathway stimulated by Shc-Grb2 association, treatment with streptozotocin leads to a tissue-specific increase in the activity of the initial step that ultimately results in the activation of the Shc/Grb2 mitogenic pathway.
Subject: Adaptor Proteins, Signal Transducing
Adaptor Proteins, Vesicular Transport
Adipose Tissue
Adrenergic Agonists
Animals
Diabetes Mellitus, Experimental
Epinephrine
Grb2 Adaptor Protein
Insulin
Liver
Muscle, Skeletal
Phosphorylation
Proteins
Rats
Receptor, Insulin
Shc Signaling Adaptor Proteins
Tyrosine
Src Homology Domains
Rights: fechado
Identifier DOI: 10.1385/ENDO:14:3:295
Address: http://www.ncbi.nlm.nih.gov/pubmed/11444425
Date Issue: 2001
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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