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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePrimary Sequence Determination Of A Kunitz Inhibitor Isolated From Delonix Regia Seeds.pt_BR
dc.contributor.authorPando, S Cpt_BR
dc.contributor.authorOliva, M Lpt_BR
dc.contributor.authorSampaio, C Apt_BR
dc.contributor.authorDi Ciero, Lpt_BR
dc.contributor.authorNovello, J Cpt_BR
dc.contributor.authorMarangoni, Spt_BR
unicamp.authorS C Pando, Department of Biochemistry, Institute of Biology, UNICAMP, 13083-970, Campinas-SP, Brazilpt_BR
unicamp.author.externalM L Oliva,pt
unicamp.author.externalC A Sampaio,pt
unicamp.author.externalL Di Ciero,pt
unicamp.author.externalJ C Novello,pt
unicamp.author.externalS Marangoni,pt
dc.subjectAmino Acid Sequencept_BR
dc.subjectChromatography, Gelpt_BR
dc.subjectChromatography, High Pressure Liquidpt_BR
dc.subjectChromatography, Ion Exchangept_BR
dc.subjectElectrophoresis, Polyacrylamide Gelpt_BR
dc.subjectFabaceaept_BR
dc.subjectMolecular Sequence Datapt_BR
dc.subjectPeptidespt_BR
dc.subjectPlant Proteinspt_BR
dc.subjectPlants, Medicinalpt_BR
dc.subjectSeedspt_BR
dc.subjectSequence Homology, Amino Acidpt_BR
dc.subjectTrypsin Inhibitorspt_BR
dc.description.abstractA serine proteinase inhibitor was purified from Delonix regia seeds a Leguminosae tree of the Caesalpinioideae subfamily. The inhibitor named DrTI, inactivated trypsin and human plasma kallikrein with K(i )values 2.19x10(-8) M and 5.25 nM, respectively. Its analysis by SDS-PAGE 10-20% showed that the inhibitor is a protein with a single polypeptide chain of M(r) 22 h Da. The primary sequence of the inhibitor was determined by Edman degradation, thus indicating that it contained 185 amino acids and showed that it belongs to the Kunitz type family; however, its reactive site did not contain Arg or Lys at the putative reactive site (position 63, SbTI numbering) or it was displaced when compared to other Kunitz-type inhibitors.en
dc.relation.ispartofPhytochemistrypt_BR
dc.relation.ispartofabbreviationPhytochemistrypt_BR
dc.date.issued2001-Julpt_BR
dc.identifier.citationPhytochemistry. v. 57, n. 5, p. 625-31, 2001-Jul.pt_BR
dc.language.isoengpt_BR
dc.description.volume57pt_BR
dc.description.firstpage625-31pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0031-9422pt_BR
dc.identifier.doipt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/11397427pt_BR
dc.date.available2015-11-27T12:29:18Z-
dc.date.accessioned2015-11-27T12:29:18Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:29:18Z (GMT). No. of bitstreams: 1 pmed_11397427.pdf: 469631 bytes, checksum: 2af6548612f0cbe029d3809a2413a81f (MD5) Previous issue date: 2001en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195017-
dc.identifier.idPubmed11397427pt_BR
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