Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194993
Type: Artigo de periódico
Title: Escherichia Coli Strains From Edema Disease: O Serogroups, And Genes For Shiga Toxin, Enterotoxins, And F18 Fimbriae.
Author: da Silva, A S
Valadares, G F
Penatti, M P
Brito, B G
da Silva Leite, D
Abstract: The objectives of the research were to determine the presence of the gene sequences for Shiga Toxin 2e (Stx2e), enterotoxins (ST-I, ST-II and LT-I), and F18 fimbriae in 144 Escherichia coli strains isolated from pigs with edema disease; to assess the ability of stx2e(+) strains to produce Stx2e; and to determine the O serogroups of the E. coli strains. Presence of the genes was determined by polymerase chain reaction (PCR), production of Stx2e was assessed by cytotoxicity for Vero and Hela cells, O serogroups were identified by agglutination with specific antisera. Of the 144 strains tested, 99 were stx2e(+) by PCR, but only 45 of these were Stx2e(+) in the cell culture assays. Among the 99 stx2e(+) strains, PCR detected the genes for F18ab, ST-I, ST-II, LT-I in 76, 40, 31 and 16 strains, respectively. Forty-one of the 99 sxt2e(+) strains belonged to O group 139; the rest did not belong to the classical edema disease O serogroups. It is likely that the enterotoxins, whose genes were detected at high frequency, are responsible for diarrhea seem in pigs with edema disease in Brazil.
Subject: Agglutination Tests
Animals
Brazil
Cercopithecus Aethiops
Edema Disease Of Swine
Escherichia Coli
Escherichia Coli Infections
Fimbriae, Bacterial
Hela Cells
Humans
O Antigens
Polymerase Chain Reaction
Shiga Toxin 2
Shiga Toxins
Vero Cells
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/11337138
Date Issue: 2001
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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