Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194985
Type: Artigo de periódico
Title: Phylogenetic Relationships Of Xylella Fastidiosa Strains From Different Hosts, Based On 16s Rdna And 16s-23s Intergenic Spacer Sequences.
Author: Mehta, A
Rosato, Y B
Abstract: The phylogenetic relationships of Xylella fastidiosa strains isolated from different hosts, including citrus trees, coffee, grapevine, plum and pear, were inferred by sequence analysis of the 16S rDNA and 16S-23S intergenic spacer region. A high level of similarity (97.1-100%) was found in the 16S rDNA of the Xylella fastidiosa strains. The 16S-23S region showed a higher level of variation, with similarity values ranging from 79.8 to 100%. Two tRNAs (tRNA(Ala) and tRNA(Ile)) were encountered within the spacer sequence. The phylogenetic trees, constructed using the neighbour-joining method, showed that the citrus, coffee, peach and plum strains were closely related and separate from grapevine strains. The pear strain remained isolated from all the other Xylella strains in both analyses and produced values of less than 20% in DNA-DNA hybridization experiments with a citrus strain. These results show that this strain does not belong to the Xylella fastidiosa genomic species.
Subject: Dna, Bacterial
Dna, Ribosomal Spacer
Gammaproteobacteria
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
Plant Diseases
Pseudomonadaceae
Rna, Ribosomal, 16s
Rna, Ribosomal, 23s
Sequence Analysis, Dna
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/11321075
Date Issue: 2001
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_11321075.pdf290.91 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.