Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194979
Type: Artigo de periódico
Title: Hydroxyurea Promotes The Reduction Of Spontaneous Bfu-e To Normal Levels In Ss And S/beta Thalassemic Patients.
Author: Bincoletto, C
Perlingeiro, R C
Saad, S T
Costa, F F
Queiroz, M L
Abstract: We have studied the effects of hydroxyurea on growth and differentiation of early erythroid progenitor cells (BFU-e) from peripheral blood of sickle cell disease patients (five SS and two Hb S/beta-thalassemia) in the presence or absence of exogenous stimulating factors. When the mononuclear cells from the sickle cell disease patients were cultured at diagnosis (before hydroxyurea treatment), there was an increased number of BFU-e in relation to controls (p < 0.05, Wilcoxon test) when cells were grown in the presence or absence of 5637 conditioned medium and erythropoietin. Colonies that developed in the absence of added growth factors were considered spontaneous. A significant difference was observed after hydroxyurea treatment in the number of BFU-e obtained in the presence and absence of stimulus, with a higher reduction in the spontaneous BFU-e number. As expected, there was an increased Hb F level in these patients when compared with their pretreatment levels. There was no correlation between spontaneous BFU-e and hemoglobin levels in all patients studied.
Subject: Anemia, Sickle Cell
Antisickling Agents
Blood Cell Count
Cell Differentiation
Cell Division
Cells, Cultured
Culture Media, Conditioned
Erythroid Precursor Cells
Erythropoietin
Heterozygote
Humans
Hydroxyurea
Sickle Cell Trait
Beta-thalassemia
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/11300342
Date Issue: 2001
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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