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Type: Artigo de periódico
Title: Eae-negative Attaching And Effacing Escherichia Coli From Piglets With Diarrhea.
Author: Penteado, A S
Aidar, L
Pestana de Castro, A F
Yamada, A
Andrade, J R
Blanco, J
Blanco, M
Blanco, J E
Abstract: One hundred and ninety strains of Escherichia coli that were isolated from pigs with diarrhea in the state of São Paulo, Brazil, and that were negative for enterotoxins and cytotoxins were investigated. Strains which adhered to HeLa cells were examined for fluorescence actin staining (FAS), the ability to induce attaching and effacing (A/E) lesions on HEp-2 cells detectable by transmission electron microscopy and the presence of eae gene sequences detected by PCR. Intimin production was detected by western blot and serogrouping was performed. Forty-seven isolates adhered to HeLa cells in several patterns, but none adhered in a localized adherence pattern. However, seven of the 47 adherent strains were positive for the FAS reaction, although the reactions were usually weak or atypical. One FAS-negative and three FAS-positive strains, which were examined for their ability to induce A/E lesions, were all positive. Subsequently, testing of these strains for the eae gene showed that they all lacked this gene. These findings, along with earlier reports of eae-negative A/E E. coli, suggest that higher quantities of E. coli in this category might be detected if more reliance were placed on phenotypic tests rather than on gene detection tests alone.
Subject: Adhesins, Bacterial
Bacterial Adhesion
Bacterial Outer Membrane Proteins
Blotting, Western
Carrier Proteins
Cercopithecus Aethiops
Escherichia Coli
Escherichia Coli Infections
Escherichia Coli Proteins
Hela Cells
Microscopy, Electron
Polymerase Chain Reaction
Shiga Toxins
Swine Diseases
Vero Cells
Citation: Research In Microbiology. v. 152, n. 1, p. 75-81
Rights: fechado
Identifier DOI: 
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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