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Type: Artigo de periódico
Title: Isolation And Characterization Of Isolectins From Talisia Esculenta Seeds.
Author: Freire, M G
Machado, O L
Smolka, M B
Marangoni, S
Novello, J C
Macedo, M L
Abstract: Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (Mr 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates.
Subject: Amino Acid Sequence
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Hemagglutination Tests
Isoelectric Focusing
Molecular Sequence Data
Molecular Weight
Plant Lectins
Citation: Journal Of Protein Chemistry. v. 20, n. 6, p. 495-500, 2001-Aug.
Rights: fechado
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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