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Type: Artigo de periódico
Title: Suramin Inhibits Respiration And Induces Membrane Permeability Transition In Isolated Rat Liver Mitochondria.
Author: Bernardes, C F
Fagian, M M
Meyer-Fernandes, J R
Castilho, R F
Vercesi, A E
Abstract: Suramin, a polysulfonated naphthylamine, caused a dose dependent inhibition of carbonyl cyanide p-(tri-fluoromethoxy)phenylhydrazone-stimulated respiration supported either by succinate or a cocktail of alphaketoglutarate, malate and isocitrate in isolated rat liver mitochondria. The half-maximum effect was obtained at 40 and 140 microM suramin for NADH- or FADH(2)-linked substrates, respectively. The respiration supported by N,N,N'N'-tetramethyl-p-phenylenediamine oxidation was unaffected by suramin (<or=500 microM). The latter respiratory substrate was used in the studies at examining the effects of suramin on the permeability properties of the inner mitochondrial membrane. These experiments provided evidence that suramin causes Ca(2+)-dependent mitochondrial swelling sensitive to the mitochondrial permeability transition (MPT) inhibitors cyclosporin A, ADP and Mg(2+). In addition, suramin decreased the content of reduced mitochondrial membrane protein thiols suggesting that thiol oxidation is the mechanism underlying suramin-induced MPT.
Subject: Adenosine Diphosphate
Carbonyl Cyanide P-trifluoromethoxyphenylhydrazone
Cell Membrane Permeability
Cell Respiration
Chelating Agents
Drug Interactions
Egtazic Acid
Enzyme Inhibitors
Intracellular Membranes
Membrane Potentials
Mitochondria, Liver
Mitochondrial Swelling
Oxygen Consumption
Rats, Wistar
Sulfhydryl Reagents
Trypanocidal Agents
Citation: Toxicology. v. 169, n. 1, p. 17-23, 2001-Dec.
Rights: fechado
Identifier DOI: 
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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