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Type: Artigo de periódico
Title: Marchantia Polymorpha Mitochondrial Orf Identifies Transcribed Sequence In Angiosperm Mitochondrial Genome.
Author: Siqueira, S F
Dias, S M
Lejeune, B
de Souza, A P
Abstract: Heterologous hybridizations performed using nine Marchantia polymorpha mitochondrial orfs and the sdh4 gene against angiosperm mtDNA suggested that three of them and the sdh4 gene have been conserved in the mitochondrial genome of different angiosperm species. Solanum tuberosum mtDNA fragments, which hybridized to M. polymorpha orf207 and sdh4 gene, were cloned, sequenced, and their expressions evaluated by Northern and RT-PCR. Hybridizing fragments to sdh4 gene and orf207 from potato mtDNA were shown to be transcribed, but only in the case of sdh4 gene was there homology between the protein encoded by the DNA sequence from M. polymorpha and the potato mitochondrial genome. M. polymorpha orf207 showed little similarity to an open reading frame from potato mtDNA, named here orf78. The putative proteins encoded by both orf207 and orf78 were not related, indicating that these orfs do not constitute homologous sequences.
Subject: Amino Acid Sequence
Arabidopsis Proteins
Base Sequence
Dna, Mitochondrial
Genome, Plant
Membrane Proteins
Molecular Sequence Data
Nucleic Acid Hybridization
Open Reading Frames
Plant Proteins
Restriction Mapping
Solanum Tuberosum
Rights: fechado
Identifier DOI: 
Date Issue: 2001
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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