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Type: Artigo de periódico
Title: Neutralization Of The Pharmacological Effects Of Bothropstoxin-i From Bothrops Jararacussu (jararacuçu) Venom By Crotoxin Antiserum And Heparin.
Author: Oshima-Franco, Y
Leite, G B
Silva, G H
Cardoso, D F
Hyslop, S
Giglio, J R
da Cruz-Höfling, M A
Rodrigues-Simioni, L
Abstract: Bothropstoxin-I (BthTX-I), the principal myotoxin of Bothrops jararacussu venom, is devoid of phospholipase A(2) (PLA(2)) activity but capable of blocking neuromuscular transmission in mouse nerve-muscle preparations. In this study, the ability of crotoxin antiserum and heparin in preventing the neurotoxic and myotoxic effects of BthTX-I was investigated. Phrenic nerve-diaphragm preparations (PND) stimulated indirectly with supramaximal stimuli (0.2 ms, 0.1 Hz) were incubated with BthTX-I (20 microg/ml) alone or with BthTX-I preincubated with antiserum or heparin for 30 min at 37 degrees C prior to testing. Control preparations were incubated with Tyrode solution, antiserum or heparin alone. BthTX-I (20 microg/ml) produced 50% neuromuscular blockade in the PND preparations in 31+/-4min, with complete blockade occurring in 120 min. The antiserum and heparin significantly prevented the neuromuscular blockade caused by BthTX-I (84 +/- 4% and 100% protection, respectively). Light microscopy examination of the muscles at the end of the 120 min incubation showed that BthTX-I damaged 48 +/- 6% of the fibers. Preincubating the toxin with antivenom significantly reduced the extent of this damage (only 15 +/- 4% of fibers affected, corresponding to 69% protection, P<0.01) whereas heparin offered no protection (34 +/- 7% of fibers affected, not significantly different from that seen with toxin alone). These results show that the antivenom was more effective in neutralizing the myotoxic effects of BthTX-I than was heparin.
Subject: Animals
Crotalid Venoms
Electric Stimulation
Electrophoresis, Polyacrylamide Gel
Immunoglobulin G
In Vitro Techniques
Injections, Subcutaneous
Muscle, Skeletal
Neuromuscular Blockade
Neuromuscular Junction
Neutralization Tests
Phrenic Nerve
Time Factors
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 39, n. 10, p. 1477-85, 2001-Oct.
Rights: fechado
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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