Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194771
Type: Artigo de periódico
Title: Morphological Aspects Of Cultured Rat Seminiferous Tubules In The Presence Of Fetal Calf Serum And Follicle-stimulating Hormone.
Author: Lombello, C B
Wada, M L
Dolder, H
Abstract: Culturing seminiferous tubules allows the analysis of spermatogenesis under controlled conditions. Reproducing the specific microenvironment for germ cells is a challenge taken up by various studies. The difficulty in supplementing all nutrients and the physical disturbances during isolation procedures cause degeneration of many cells in the seminiferous epithelium. We tested some culture conditions in order to preserve an acceptable morphology of cells inside the tubules. Seminiferous tubules were cultured during fifteen days in HAM F10 medium supplemented with fetal calf serum (FCS) and/or follicle-stimulating hormone (FSH). The cellular morphology was analyzed using scanning and transmission electron microscopy. During the culture period cellular degeneration occurred progressively. Morphological modifications of the Sertoli cells and germ cells such as accumulation of lipid droplets, nuclear and cytoplasmatic vacuolization and the presence of cell debris were observed. The addition of FCS activated the myoid cells causing nuclear rounding and thickening of the tubular wall. The best results were obtained with a serum-free culture medium supplemented with FSH.
Subject: Animals
Blood
Culture Media
Follicle Stimulating Hormone
Male
Microscopy, Electron
Microscopy, Electron, Scanning
Organ Culture Techniques
Rats
Rats, Wistar
Seminiferous Tubules
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/10877108
Date Issue: 2000
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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