Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194767
Type: Artigo de periódico
Title: A Quantitative Enzyme-linked Immunosorbent Assay (elisa) For The Immunodiagnosis Of Neurocysticercosis Using A Purified Fraction From Taenia Solium Cysticerci.
Author: da Silva, A D
Quagliato, E M
Rossi, C L
Abstract: A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis is described. The ELISA was standardized using a purified Taenia solium cysticerci fraction (PCF) obtained by ion exchange chromatography. The ELISA using PCF (PCF-ELISA) and a qualitative ELISA using a whole extract from T. solium cysticerci (WEC-ELISA) were used to screen for cysticercus-specific IgG antibodies in cerebrospinal fluid (CSF) samples from 57 patients with neurocysticercosis and 50 patients with other neurologic disorders. The sensitivity of both assays was 95%, whereas the specificities of PCF-ELISA and WEC-ELISA were 100% and 92%, respectively. The excellent sensitivity and specificity of the PCF-ELISA make this assay a potentially useful tool in screening for antibodies against T. solium cysticerci.
Subject: Animals
Antibodies, Helminth
Antigens, Helminth
Chromatography, Ion Exchange
Cysticercus
Enzyme-linked Immunosorbent Assay
Humans
Neurocysticercosis
Sensitivity And Specificity
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/10863102
Date Issue: 2000
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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