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Type: Artigo de periódico
Title: Adsorption Of Inulinases In Ion-exchange Columns.
Author: Silva, F R
Santana, C C
Abstract: The use of adsorption columns packed with ion-exchange resins for recovering, concentrating and purifying proteins is now widespread. The present work consists of a study on the dynamic behavior of adsorption columns that uses two kinds of adsorbents: a cationic and an anionic resin. A frontal analysis of the columns was performed with experimental data obtained from Fructozyme, a mixture of inulinase enzymes. The parameters of a Langmuir type of isotherm and adsorption kinetics were obtained from experimental tests in a batch system. A numerical technique based on orthogonal collocation and a fourth-order Runge-Kutta method was coupled with a nonlinear optimization method to predict the coefficients of the rate equations, which are fundamental for scale-up purposes.
Subject: Adsorption
Aspergillus Niger
Chromatography, Ion Exchange
Glycoside Hydrolases
Ion Exchange Resins
Citation: Applied Biochemistry And Biotechnology. v. 84-86, p. 1063-78, 2000.
Rights: fechado
Identifier DOI: 
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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