Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/194744
Type: Artigo de periódico
Title: Cyclosporin A Inhibits Production And Activity Of Matrix Metalloproteinases By Gingival Fibroblasts.
Author: Bolzani, G
Della Coletta, R
Martelli Júnior, H
Graner, E
Abstract: Cyclosporin A (CyA) is a potent immunosuppressor used in organ transplantation and in the management of various autoimmune diseases. Gingival overgrowth is one of the side-effects of the CyA-treatment, affecting the attached gingiva of 25-81% of treated patients. To investigate the production and activity of matrix metalloproteinases (MMPs) in the CyA-induced gingival overgrowth, 2 well-documented models were utilized: the in vivo CyA-induced rat gingival overgrowth and primary cultures of human gingival fibroblasts treated with CyA. Our results obtained from the Western blot assays demonstrated clearly that the production of MMP-1 and MMP-3 was significantly inhibited by CyA at similar concentrations found in the serum of patients undergoing CyA-treatment. Moreover, the gelatinolytic activity of MMP-2 was also reduced both in cultured fibroblasts and in the rat CyA-induced gingival overgrowth. Taken together, the data presented here suggest that these inhibitory effects may contribute to the extracellular matrix (ECM) components accumulation in the CyA-induced gingival overgrowth.
Subject: Adult
Analysis Of Variance
Animals
Blotting, Western
Cells, Cultured
Cyclosporine
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix
Female
Fibroblasts
Gingiva
Gingival Overgrowth
Humans
Male
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinases
Rats
Rats, Wistar
Rights: fechado
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/10791709
Date Issue: 2000
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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